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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chifefabmeric vectors into 293T cells and harvest of supernatants 48 h later。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cell for FeFAB assay were kind gifts from Dr. Martin Lochelt, German Cancer Research Center, Germany.

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